Immunogen
Synthetic peptide of amino acids surrounding the phosphoSerine 58 site of rat 14-3-3 Protein.
Application
Anti-14-3-3 phospho Serine58 Antibody detects level of 14-3-3 phospho Serine58 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 1:1,000
Biochem/physiol Actions
14-3-3 Protein, phosphoSerine58. The antibody recognizes a protein of ~29 kDa corresponding to 14-3-3 Protein, phosphoSerine58 in lysates from rat brainstem. Immunolabeling is blocked by the phosphopeptide used as the immunogen but not the corresponding non-phosphopeptide.
The immunogen sequence has 100% conservation with mouse and Xenopus.
Physical form
ImmunoAffinity Purified
Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Preparation Note
Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_006761.3 |
| UniProt accession no. | P62258 |
| shipped in | dry ice |
| target post-translational modification | phosphorylation (pSer58) |
| Gene Information | human ... YWHAE(7531) |
