General description
105 kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 850-865 (GLPTDRDLGGACPPQD) of human protein kinase D2 (PKD2).
Application
Anti-PKD2 Antibody detects level of PKD2 & has been published & validated for use in WB.
Immunoblot Analysis: A 1:500-1:1,000 dilution of this antibody detected PKD2 in RIPA lysates from A431 cells that had been stimulated with PMA.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Biochem/physiol Actions
Recognizes PKD2.
Physical form
ImmunoAffinity Purified
Immunoaffinity purified rabbit IgG in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl and 0.05% sodium azide as a preservative.
Preparation Note
Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
PMA stimulated A431 cells
Routinely evaluated by immunoblot in RIPA lysates from HEK293m cells that had been stimulated with 50 ng/mL of PDBu for 10 minutes or PMA stimulated A431 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_000297 |
| UniProt accession no. | Q9BZL6 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PKD2(353503) |
