The CD34 MicroBead Kit has been developed for the positive selection of CD34+ hematopoietic stem and progenitor cells in a single step. CD34+ cells can be isolated from:
peripheral blood (PBMCs)
bone marrow1
leukapheresis product2
cord blood3
differentiated ES and iPS cell lines4,5.
Detailed product information
Background information
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts5,6, endothelial progenitor cells5,7-9, and mature endothelial cells8.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.
Downstream applications
Cells isolated with the CD34 MicroBead Kit are suitable for various downstream applications, including:
flow cytometric analysis
expansion in culture
in vitro differentiation5
reprogramming3
retroviral transduction10
gene expression analyses
transplantation into humanized mice
Columns
MS, LS, XS, or autoMACS® Columns.
