Application
Immunohistochemistry on acetone fixed cryostat sections: 0.5-10 μg/mL using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 {higher triton is only used on thick sections; blocked for 1-3 hours at room temperature. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4C. Washed 3x10′ in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites.
Immunocytochemistry on cell smears: 0.5-10 μg/mL.
Western blot: MnS1 antibody will recognize at 25kDa band under reducing conditions; this is the monomer of the MnSOD protein.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
This Anti-MnSOD Antibody, clone MnS-1 is validated for use in WB, IC, IH for the detection of MnSOD.
Biochem/physiol Actions
Antibody reacts with MnSOD
Physical form
Format: Purified
Purified immunoglobulin. Liquid in PBS.
Preparation Note
Prior to opening maintain at 2-8°C for up to six month after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MnS-1, monoclonal |
| species reactivity | rat, rabbit, human |
| should not react with | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_000636.2,NM_001024465.1,NM_001024466.1 |
| UniProt accession no. | P04179 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SOD2(6648) |
