Immunogen
Purified peptide from amino acids 581-595 of rat Cav3.2
Application
Anti-Cav3.2 Antibody is an antibody against Cav3.2 for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Signaling Neuroscience
Western blot: 1:200 using ECL on ND7/23 cell lysate (mouse neuroblastoma x rat DRG neuron hybrid cell line).
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Cav3.2 (α1H (T-Type)).
The immunogen sequence is 14 of 15 amino acids identical in mouse, bovine and canine and 13 of 15 amino acids identical in human.
Physical form
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
ImmunoAffinity Purified
Preparation Note
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| isotype | IgG |
| UniProt accession no. | O95180,Q9EQ60 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | rat ... Cacna1H(114862) |
