General description
C-C chemokine receptor type 6 (UniProt: P51684; also known as C-C CKR-6, CC-CKR-6, CCR-6, Chemokine receptor-like 3, CKR-L3, DRY6, G-protein coupled receptor 29, GPR-CY4, GPRCY4, LARC receptor) is encoded by the CCR6 (also known as CKRL3, CMKBR6, GPR29, STRL22) gene (Gene ID: 1235) in human. The gene is mapped to human chromosome 6q27. It shares sequence similarity with G protein-coupled chemokine receptor. CCR6 plays a crucial role in the immune system by mediating the migration and positioning of various immune cells. CCR6 encodes a chemokine receptor that is mainly expressed in B cells and memory T cells. It is also found to be expressed in dendritic cells, spleen, thymus, small intestine, and peripheral blood leukocytes. It is primarily localized on the plasma membrane of cells, where it interacts with its ligand CCL20. CCL20 is the only known high-affinity ligand that binds to CCR6. Upon ligand binding, CCR6 undergoes conformational changes that activate intracellular signaling pathways, leading to cellular responses such as chemotaxis. CCR6 has been implicated in various diseases, particularly those involving inflammatory and autoimmune responses. Overexpression of CCR6 has been observed in certain cancers, such as colorectal cancer and hepatocellular carcinoma. Studies show a very strong correlation between the expression of CCR6 and disease severity in patients with inflammatory bowel disease (IBD), psoriasis (PS), rheumatoid arthritis (RA), and multiple sclerosis (MS). CCR6 could be a potential therapeutic target in these diseases and, CCR6 antibodies could be a promising alternative to small molecules in the treatment of CCR6/CCL20 axis-related pathologies. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gómez-Melero, S., et al. (2023). Antibodies (Basel). 12(2): 30; Meitei. HT., et al. (2021). Autoimmun Rev. 20(7):102846).
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from an extracellular domain of human CCR6/CD196.
Application
Quality Control Testing
Evaluated by Western Blotting in HuH-7 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CCR6/CD196 in HuH-7 cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CCR6/CD196 in human spleen tissue sections.
Flow Cytometry Analysis: 1 µg from a representative lot detected CCR6/CD196 in human peripheral blood mononuclear cells (PBMC).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CCR6/CD196 in HL-60 cells.
Affinity Binding Assay: A representative lot of this antibody bound CCR6/CD196 peptide with at least one hundred thousand-fold (100,000X) higher than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1K9 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects CCR6/CD196. It targets an epitope within 16 amino acids from the third extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1K9, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 42.49 kDa,observed mol wt ~57 kDa |
| purified by | using Protein A |
| species reactivity | human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | Internal |
| Protein ID accession no. | NP_004358 but on blast NP_001381511 |
| UniProt accession no. | P51684 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... CCR6(1235) |
