General description
Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.
~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.
Immunogen
GST-tagged recombinant protein corresponding to mouse MyoD.
Application
Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.
Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.
Research Category
Signaling
Research Sub Category
Developmental Signaling
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
C2C12 cell lysate
Evaluated by Western Blot in C2C12 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE514
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 5F11, monoclonal |
| species reactivity | mouse |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_034996 |
| UniProt accession no. | P10085 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Myod1(17927) |
