General description
抗-Hsp47,小鼠单克隆,克隆M16.10A1,可识别多种物种中~47 kDa Hsp47蛋白。经验证可用于ELISA、EIA、WB、ICC、IF、IP和IHC(石蜡切片)。
蛋白G纯化的小鼠单克隆抗体。识别~47 kDa Hsp47蛋白。
识别~47 kDa Hsp47蛋白。
Immunogen
大鼠
来自L6细胞的Hsp47
Application
ELISA(见备注)
酶免疫测定(见备注)
免疫印迹(1 µg/ml,比色)
免疫细胞化学(1 µg/ml)
免疫荧光(5 µg/ml)
免疫沉淀(5 µg/ml)
石蜡切片(见备注)
Packaging
请参考特定浓度批号的标签。
Physical form
在PBS,50%甘油,pH 7.2中。
Preparation Note
初次融化后,等分并冷冻(-20°C)。
Analysis Note
阳性对照
3T3细胞,,人乳腺癌组织
Other Notes
Kuroda, K., et al. 1998.J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998.Exp.Cell Res.179, 289.
Clark, E.P., et al. 1991.J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A.1991.Biochem.J.274, 793.
Cates, G.A., et al. 1987.Biochem.Cell Biol. 65, 767.
Saga, S., et al. 1987.J. Cell Biol.105, 517.
Nagata, K., et al. 1986.J. Biol. Chem. 261, 7531.
据报道,该抗体也可用于ELISA和酶免疫测定。合适的稀释度由与测定条件相关的变量决定。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
| biological source | mouse |
| Quality Level | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | M16.10A1, monoclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | mouse, porcine, chicken, canine, human, hamster, guinea pig, bovine, rat, monkey, sheep |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (ELISA Enzyme Immunoassay Immunoblotting (1 µg/mL, colorimetric) Immunocytochemistry (1 µg/mL) Immunofluorescence (5 µg/mL) Immunoprecipitation (5 µg/mL) Paraffin Sections ) |
| isotype | IgG2b |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... SERPINH1(871) |

