规格
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Crosslinker Type:
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Heterobifunctional |
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Labeling Method:
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Chemical Labeling |
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Product Line:
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Pierce™ |
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Reactive Moiety:
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Haloacetyl, NHS Ester |
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Spacer:
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Short |
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Solubility:
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DMSO (Dimethylsulfoxide), DMF (Dimethylformamide) |
储存
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Upon receipt store at 4°C protected from moisture. Product is shipped at ambient temperature. |
Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
Features of SIA:
• Reactive groups: NHS ester and iodoacetyl
• Reactive toward: amino and sulfhydryl groups
• Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB
• NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond
• Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
