General description
The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
Application
This Anti-CD80 (mouse), APC, clone 16-10A1 Antibody is validated for use in Flow Cytometry for the detection of CD80.
Analysis Note
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 μg from a representative lot detected CD80 in one million C57Bl/6 splenocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | hamster (Armenian) |
| Quality Segment | 100 |
| conjugate | Allophycocyanin conjugate |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 16-10A1, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable |
| isotype | IgG |
| UniProt accession no. | Q00609 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Cd80(12519) |
