General description
SUMO-activating enzyme subunit 2, also known as Anthracycline-associated resistance ARX, or Ubiquitin-like 1-activating enzyme E1B, and encoded by the gene name UBA2 or SAE2 or UBLE1B, belongs to the ubiquitin-activating E1 family. SUMOs are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.
~80 kDa observed. The calculated molecular weight is 72 kDa, however SAE2 has been shown as a ~70-90 kDa band in western blots (Truong, K., et al. (2012). J. Biol. Chem. 287(51):42611-42619).
Immunogen
GST-tagged recombinant protein corresponding to human SAE2.
Application
Anti-SAE2 Antibody, clone 7H8.1 | MABE927 is an antibody against SAE2 Antibody for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SAE2 in human kidney and rat testis tissue.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Neuro-2a cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SAE2 in 10 µg of Neuro-2a cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7H8.1, monoclonal |
| species reactivity | rat, human, mouse |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_005490 |
| UniProt accession no. | Q9UBT2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UBA2(10054) |
