General description
125 kDa
Immunogen
peptide corresponding to residues 934-952 (RDELCVAGAQLYACQDPAI) of murine JAK3. The immunizing peptide sequence shares 16/19 amino acids with rat JAK3, but contains an amino acid insertion, which may block cross-reactivity. The immunizing peptide shares 12/19 amino acids with human JAK3.
Application
Anti-JAK3 Antibody is an antibody against JAK3 for use in WB.
Biochem/physiol Actions
: Recognizes murine JAK3, Mr 125kDa and JAK3 degradation products, Mr 95-120kDa. The antiserum shows very poor immunoreactivity for human JAK3. No immunoreaction was noted with immuno-precipitated mouse JAK1 and JAK2
This antibody has been tested on several human cell lines including A431, Hela, Jurkat, and HEK293 cells. The antiserum shows very poor immunoreactivity for human JAK3
Physical form
antiserum containing 0.05% sodium azide
Preparation Note
2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
routinely evaluated by immunoblot on lysate from a mouse cytotoxic T lymphocyte cell line (CTLL)
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_000215 |
| UniProt accession no. | P52333 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Jak3(16453) |
