General description
68.99 kDa calculated.
Mesothelin (UniProt: Q13421; also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, MSLN) is encoded by the MSLN (also known as MPF) gene (Gene ID: 10232) in human. Mesothelin is a glycophosphatidylinositol (GPI)-linked cell surface protein that normally expressed in lung and at lower levels in heart, placenta, and kidney. It is highly expressed in several types of malignant tumors and is associated with increased tumor aggressiveness and poor clinical outcome. Antibodies against mesothelin have been detected in patients with mesothelioma and ovarian cancer. Mesothelin plays a role in cellular adhesion. Mesothelin is synthesized with a signal peptide (aa 1-36) and a propeptide (aa 607-630) that are cleaved to produce mature mesothelin, which can be cleaved further by furin-like convertase to generate megakaryocyte-potentiating factor (aa 37-206) and the cleaved form of mesothelin (aa 296-606). Four isoforms of mesothelin have been described that are produced by alternative splicing. (Ref.: Inaguma, S., et al. (2017). Oncotarget. 8(16); 26744-26754; Onda, M., et al. (2005). Clin. Cancer Res, 11(16); 5840-5846).
Immunogen
Plasmid cDNA corresponding to the mesothelin extracellular domain followed by a single boost with a recombinant human mesothelin-Fc fusion protein.
Application
Anti-Mesothelin, clone MN-1 Antibody, Cat. No. MABC1140, is a mouse monoclonal antibody that detects Mesothelin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Mesothelin in human uterus tissue sections.
Flow Cytometry Analysis: A representative lot detected Mesothelin in Flow Cytometry applications (Onda, M., et. al. (2005). Clin Cancer Res. 11(16):5840-6).
ELISA Analysis: A representative lot detected Mesothelin in ELISA applications (Onda, M., et. al. (2005). Clin Cancer Res. 11(16):5840-6).
Western Blotting Analysis: A representative lot detected Mesothelin in Western Blotting applications (Onda, M., et. al. (2005). Clin Cancer Res. 11(16):5840-6).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected Mesothelin in Immunohistochemistry applications (Onda, M., et. al. (2005). Clin Cancer Res. 11(16):5840-6; Inaquma, S., et. al. (2017). Oncotarget. 8(16):26744-26754).
Research Category
Apoptosis & Cancer
Biochem/physiol Actions
Clone MN-1 is a mouse monoclonal antibody, generated in mesothelin-deficient mice, which specifically detects human Mesothelin.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry (Paraffin) in human fallopian tube tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Mesothelin in human fallopian tube tissue sections.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | MN-1, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | ELISA: suitable,flow cytometry: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_037536.2 |
| UniProt accession no. | Q13421 |
| target post-translational modification | unmodified |
| Gene Information | human ... MSLN(10232) |
