General description
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
This PhosphoDetect
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
Application
ELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Physical form
Lyophilized from PBS, PEG, sucrose.
Preparation Note
Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Vanadate-treated A431 cells
Other Notes
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 5C6, monoclonal |
| form | lyophilized |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | human, mouse |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (ELISA (0.05 µg/mL) Immunoblotting (1 µg/mL, colorimetric; 0.1 µg/mL, chemiluminescence) Immunocytochemistry (1-10 µg/mL) Immunoprecipitation (1-10 µg/10 vanadate-treated A431 cells)) |
| isotype | IgG1 |
| shipped in | ambient |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer157) |
| Gene Information | human ... VASP(7408) |
