Immunogen
2.5 S form of mouse NGF.
Application
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor Antibody, clone 27/21 is validated for use in ELISA for the detection of Nerve Growth Factor.
Biochem/physiol Actions
NGF is a neurotrophic protein which is synthesized by the target tissues of NGF-sensitive neurons. NGF acts on sympathetic and sensoric neurons as well as on cholinergic neurons of the basal forebrain. It has been suggested that NGF is involved in the neuropathology of Alzheimer′s Disease (Hefti & Weiner, 1986). In the peripheral nervous system, NGF plays a role in the development of autonomic and sensory neuropathies (Anand et al., 1991).
The antibody specifically reacts with the beta -subunit of NGF from mouse, in the 2.5S- as well as in the 7S-form.
The cross-reactivity with human NGF is 60-90 % of the reactivity with mouse NGF
Physical form
Format: Purified
Purified immunoglobulin. Lyophilized from 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. Reconstitute with 1 mL of sterile distilled water.
Preparation Note
The lyophilized antibody is stable if stored dry at 2-8°C for up to 12 months. After reconstitution maintain at 4°C unaliquotted but sealed tightly for 6 months; do not freeze thaw.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 27/21, monoclonal |
| species reactivity | human, rat, mouse, bovine |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001102654.1,NM_002527.4 |
| UniProt accession no. | P20783 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NTF3(4908) |
