General description
AITRL, a member of the TNF superfamily, is expressed in endothelial cells, and signals through the AITR receptor. AITRL regulates T-cell proliferation and survival, and effectuates the interaction between T lymphocytes and endothelial cells. The AITRL gene codes for a type II transmembrane protein comprised of 177 amino acids, including a 28 amino acid cytoplasmic region, a 21 amino acid transmembrane domain and a 128 amino acid extracellular domain. Recombinant human soluble AITRL is a 14.3 kDa protein, containing 126 amino acid residues corresponding to the extracellular domain of AITRL.
Biochem/physiol Actions
AITRL, a member of the TNF superfamily, is expressed in endothelial cells, and signals through the AITR receptor. Recombinant human soluble AITRL is a 14.3 kDa protein, containing 126 amino acid residues corresponding to the extracellular domain of AITRL.
Physical form
Lyophilized from 10 mM Tris, pH 8.0.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
ETAKEPCMAK FGPLPSKWQM ASSEPPCVNK VSDWKLEILQ NGLYLIYGQV APNANYNDVA PFEVRLYKNK DMIQTLTNKS KIQNVGGTYE LHVGDTIDLI FNSEHQVLKN NTYWGIILLA NPQFIS
| biological source | human |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC),≥98% (SDS-PAGE) |
| form | lyophilized |
| mol wt | 14.3 kDa |
| packaging | pkg of 20 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <0.1 EU/μg endotoxin, tested |
| color | white to off-white |
| UniProt accession no. | Q9UNG2 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... TNFSF18(8995) |
