General description
The calcium-activated potassium ion channel (KCNN3) is a voltage-independent small-conductance calcium-activated potassium channel. It is abundantly expressed in the brain and central nervous system. Its predominant expression is seen in the hypothalamus. KCNN3 gene is located on human chromosome 1q21.3.
Immunogen
synthetic peptide corresponding to amino acids 2-21 of human potassium channel SK3. The epitope is highly homologous in rat.
Application
Anti-Potassium Channel SK3 (KCa3, KCNN3, SKCa3) antibody produced in rabbit has been used in western blot analysis (1:250) and immunohistochemical staining (1:50).
Biochem/physiol Actions
Anti-Potassium channel SK3 recognizes the SK3 protein in humans and rats by immunoblotting. Potassium channels contribute to maintaining cell volume, membrane potential, neuronal excitability, and the secretion of transmitters, salt, and hormones. It is extremely sensitive to changes in intracellular calcium levels, which are common during action potentials. KCNN3 helps to determine neuronal firing rates via the after-hyperpolarization. It is a migraine candidate susceptibility gene. Aberrations in the KCNN3 gene may trigger cortical spreading depression (CSD) and trigeminal sensitivity.
Physical form
Lyophilized at ~0.6 mg/ml from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | lyophilized powder |
| species reactivity | rat, human |
| technique(s) | western blot (chemiluminescent): 1:200 using rat brain membranes |
| UniProt accession no. | Q9UGI6 |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... KCNN3(3782) rat ... Kcnn3(54263) |
