General description
Mutant of E. coli 0111:B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes. Highly purified preparation.
Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Inducible nitric oxide synthase
Product does not compete with ATP.
Reversible: no
Preparation Note
Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 1 year at 4°C.
LPS will form a suspension in H₂O; slight warming and vortexing may be required to achieve complete dispersion in H₂O. Addition of 0.5% triethylamine will also help with dispersion; triethylamine is very basic and can be neutralized with Trix-HCl to avoid hydrolysis of the fatty acid chains.
Other Notes
Hattori, S., et al. 1995. Biochem. Mol. Biol. Int.35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol.49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem.60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
| Quality Level | 100 |
| description | RTECS - OJ0895400 |
| form | lyophilized |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| impurities | <2.5% Protein,5.0-10.0% 2-keto-3-deoxyoctonate (KDO) |
| solubility | water: 1 mg/mL |
| anion traces | phosphate (PO43-): 6.0-15.0% |
| shipped in | ambient |
| storage temp. | 2-8°C |
