General description
Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.
~216 kDa observed
Immunogen
Recombinant protein corresponding to human Girdin.
Application
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate.
This Anti-Girdin, C-terminus Antibody, clone 10E6.1 is validated for use in Western Blotting, IHC(P), ICC, Immunoprecipitation for the detection of Girdin, C-terminus, clone 10E6.1.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot in human brain tissue lysate.
Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 10E6.1, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001129069 |
| UniProt accession no. | Q3V6T2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CCDC88A(55704) |
