产品介绍
Product Description
General description
N-terminal GST tagged recombinant human c-Kit, amino acids 544 - end, containing the V560G mutation. The V560G mutation has been found in patients with gastrointestinal stromal tumours and has been linked to poor prognosis. This mutation confers increased susceptibility to Gleevec
Product Source: Human c-Kit (V560G) expressed in Sf21 insect cells.
Physical form
Presented as a frozen solution in 50mM Tris/HCl pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, and 0.1% 2-mercaptoethanol.
Analysis Note
Routinely evaluated by phosphorylation of 250μM GGMEDIYFEFMGGKKK.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
技术参数
Specifications
| biological source | human |
| Quality Segment | 100,300 |
| mol wt | Mw 76.6 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| NCBI accession no. | NM_000222.2,NM_001093772.1 |
| UniProt accession no. | P10721 |
| Gene Information | human ... KIT(3815) |
