产品详情

CpGenome Rat Unmethylated Genomic DNA Standard,1SET,S7860,Sigma

销售价: ¥ 2879.34 / 件
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订货号 0BV8979
品牌型号 S7860
货期 询货期
最小订货量 1件
产品介绍 Product Description

General description

The CpGenome Rat Unmethylated Genomic DNA Standard is ideal for use as a control or standard in DNA methylation studies. This unmethylated standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase. This process results in a DNA standard which contains ≥ 95% unmethylated cytosines across the genome in both heterochromatic and euchromatic regions.


Application

Research Category
Epigenetics & Nuclear Function


Physical form

5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA


Preparation Note

Upon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.


Analysis Note

Quantitative Methylation Specific PCR (qMSP):
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.


Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


技术参数 Specifications
Quality Segment100
species reactivityrat
manufacturer/tradenameCpGenome,Upstate®
shipped inwet ice
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