General description
Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Immunogen
Phosphotyramine-KLH.
Application
Anti-Phosphotyrosine Antibody, clone 4G10
Research Category
Signaling
Research Sub Category
General Post-translation Modification
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Suitable for immunoprecipitation and affinity purification.
Biochem/physiol Actions
Recognizes tyrosine-phosphorylated proteins from all species.
Features and Benefits
Format: Gel Immobilized
Physical form
1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% preservative, for a total volume of 2 mL. Liquid suspension.
Protein A purified
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4G10®, monoclonal |
| species reactivity | vertebrates |
| manufacturer/tradename | Upstate® |
| technique(s) | immunoprecipitation (IP): suitable |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PID1(55022) |
