General description
Millipore’s Human iPS Selection Kit is a quick, easy and non-invasive method to monitor the pluripotent state of live human ESC and fully reprogrammed human iPS cells using immunocytochemistry (ICC). The Human iPS Selection Kit allows live cell imaging and identification of fully reprogrammed human iPS cells from a heterogeneous population of reprogramming intermediates and enables the live selection of human iPS cells that can be further passaged and expanded for downstream applications. The selected live stained human iPS clones maintain normal human ESC morphology and can proliferate and be passaged normally. The kit consists of the following: (1) two conjugated antibodies that are known markers of human pluripotency, Tra-I-60 and SSEA-4, (2) a negative selection marker (CD13-PE) that is specific to fibroblasts and (3) a cell permeable Hoechst nuclear dye that possesses the dual function of labeling all cells and also distinguishing partially reprogrammed cells (Hoechst Bright) from fully reprogrammed cells (Hoechst Dim) (Chan et al,).
Recent findings indicate that fully reprogrammed human iPS cells possess the following characteristics: (1) they downregulate expression of fibroblast marker, CD13 (2) they upregulate expression of pluripotent markers, SSEA-4 and TRA-1-60, (3) they silence the viral transgenes while (4) reactivating endogenous expression of Nanog and (5) they assume a HoeschstDim phenotype. These characteristics have enabled reliable identification of fully reprogrammed human iPS cells from a mixed population of partially reprogrammed cells.
Application
Research Category
Stem Cell Research
The Human iPS Selection Kit allows live cell imaging & identification of fully reprogrammed human induced pluripotent Stem cells from a heterogeneous population of reprogramming intermediates.
Packaging
Sufficient for 40 tests per antibody
Preparation Note
Stable for 6 months at 2-8°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Maintain refrigerated at 2-8°C, protected from light in undiluted aliquots.
Other Notes
Anti-SSEA-4 PE (Mouse monoclonal IgG3), Cat. No. CS204438. One vial containing 50 μg of 0.25 mg/mL purified mouse IgG antibody
Anti-TRA-1-60 FITC (Mouse monoclonal IgM), Cat. No. CS204460. One vial containing 50 μg of 0.5 mg/mL purified mouse IgM antibody
Anti-CD13 PE (Mouse monoclonal IgG1), Cat. No. CS204449. One vial containing 50 μg of 1 mg/mL purified mouse IgG antibody
Hoechst Nuclear Stain, Cat. No. CS204269. One vial containing 15 μL of 1 mg/mL stock solution.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| Quality Segment | 100 |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | cell culture | stem cell: suitable,immunocytochemistry: suitable |
| input | sample type: human embryonic stem cell(s) sample type induced pluripotent stem cell(s) |
| shipped in | wet ice |
| storage temp. | 2-8°C |
