General description
The protein named Angiopoietin-related protein 2 (ANGPTL2) or Angiopoietin-like protein 2 and encoded by the human gene named ANGPTL2/ARP2 is a secreted protein that is highly expressed in several tissues including the heart, spleen, stomach, colon, ovary and skeletal muscle. ANGPTL2 functions to induce endothelial cell sprouting in preparation for angiogenesis. ANGPTL2 is produced by endothelial cells and ANGPTL2 appears to function as an autocrine in helping to promote the sprouting response. ANGPTL2 also appears to use the same autocrine function in certain cancers and in particular in driving of metastasis. Recent research have demonstrated that tumor cell–derived ANGPTL2 increased in vitro motility and invasion in an autocrine/paracrine manner, conferring an aggressive metastatic tumor phenotype on the cells examined. Thus research is now focused on blocking the function of ANGPTL2 as one possible treatment for metastatic cancers. EMD-Millipore’s Anti-ANGPTL2 rabbit polyclonal antibody has been tested in western blot on mouse small intestine tissue extracts and in paraffin embedded immunohistochemistry on human heart tissue sections.
~60 kDa observed
Immunogen
N-Trx, C-6XHis-tagged recombinant protein corresponding to human ANGPTL2.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ANGPTL2 in human heart sections tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-ANGPTL2 antibody is validated for use in WB, IH for the detection of ANGPTL2.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in transfected HEK293 with ANGPTL2.
Western Blotting Analysis: A 1:250 dilution of this antibody detected ANGPTL2 in 10 µg of transfected HEK293 with ANGPTL2.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_036230 |
| UniProt accession no. | Q9UKU9 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ANGPTL2(23452) |
