General description
Monoclonal Anti-Connexin 43 (mouse IgM isotype) is derived from the hybridoma CXN-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide from the C-terminal region of Connexin 43 protein, conjugated to KLH. Connexin 43 protein (Cx43), also known as Gap junction α-1 protein or Gap junction 43 kDa heart protein. It has four transmembrane domains (TMs). The N and C-terminal cytoplasmic tails orient in plasma membrane. It also has two extracellular loop (EL) domains and a cytoplasmic loop (CL). Six connexin subunits form a connexon or hemichannel in the plasma membrane and a head-to-head docking between two hemichannels result in the formation of a gap junction channel. Cx43 is expressed in heart and brain. It is expressed in cell types including the melanocytes, keratinocytes, endothelial and basal cells. Cx43 has been mapped to human chromosome 6q22.31.
Immunogen
Synthetic peptide corresponding to human connexin-43 C-terminal region, conjugated to KLH
Application
The antibody may be used in various immunochemical techniques including Immunoblot (~43 kDa), Immunohistochemistry and Immunofluorescence.
Biochem/physiol Actions
Connexin 43 (Cx43) functions as a neuroprotector and is most prominently expressed in astrocytes and microglial cells in the brain. It plays a key role in intercellular communication. Cx43 mediates synchronized contraction of the heart. It modulates of the cell migration and cell adhesion processes. Altered connexin 43 expression is implicated in hypertrophic cardiomyopathy. It is less expressed in human tumors. It is a potential target for treating skin disorders. Its downregulation favours wound healing.
Monoclonal Anti-Connexin 43 specifically recognizes Connexin 43 from human1,2, mouse2, rat3, porcine4, chicken5 and feline6 origin.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | CXN-6, monoclonal |
| mol wt | ~43 kDa |
| species reactivity | porcine, feline, chicken, mouse, human, rat |
| packaging | antibody small pack of 25 μL |
| concentration | ~1 mg/mL |
| technique(s) | immunoblotting: 0.5-1 μg/mL,immunofluorescence: 10-20 μg/mL |
| isotype | IgM |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | Porcine ... GJA1(100518636) chicken ... GJA1(395278) feline ... GJA1(101100211) human ... GJA1(2697) mouse ... GJA1(14609) rat ... GJA1(24392) |
