产品详情

Origami B Competent Cells - Novagen,0.4ML,70836-3CN,Sigma

销售价: ¥ 1800.54 / 件
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订货号 0BV4307
品牌型号 70836-3CN
货期 询货期
最小订货量 1件
产品介绍 Product Description

General description

Genotype: F-ompT hsdSB(rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 trxB (KanR, TetR)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Origami B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner and Origami hosts in one strain background. The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, these strains are recommended for use only with pET plasmids carrying the ampicillin resistance marker bla.
Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.


Other Notes

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlOrigami B Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid


Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)


技术参数 Specifications
biological sourceEscherichia coli
Quality Level100
manufacturer/tradenameNovagen®
storage conditionOK to freeze
growth modeadherent or suspension
morphologyrod shaped
technique(s)microbiological culture: suitable
cell transformationtransformation efficiency: >2×106 cfu/μg
shipped indry ice
storage temp.−70°C
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