General description
41 kDa calculated
HLA-C, also known as HLA class I histocompatibility antigen, Cw-1 alpha chain, also known as MHC class I antigen Cw*1, and encoded by the gene HLA-C/HLAC. It contains an alpha and beta (beta-2-microglobulin) chain and interacts with the human herpesvirus 8 MIR1 protein.
The previously assigned protein identifier P30499 has been merged into P10321. Full details can be found on the UniProt database.
Immunogen
Purified tamarin MHC class I molecules corresponding to human HLA-C.
Application
Anti-HLA-C Antibody, clone DT9 is an antibody against HLA-C for use in flow cytometry & IP.
Flow Cytometry Analysis: A representative lot from an independent laboratory detected HLA-C in peripheral blood CD3+ cells from 200 healthy human donors (Apps, R., et al. (2013). Science. 340(6128):84-91.).
Flow Cytometry Analysis: A representative lot from an independent laboratory detected HLA-C in human PBMCs (Corrah, T. W., et al. (2011). J Virol. 85(7):3367-3374.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HLA-C from various human cell lines (Braud, V. M., et al. (1998). Curr Biol. 8(1):1-10.).
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Flow Cytometry in human PBMCs.
Flow Cytometry Analysis: 1 µg of this antibody detected HLA-C in 1X10E6 human PBMCs.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | DT9, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2bκ |
| UniProt accession no. | P10321 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HLA-C(3107) |
