General description
Hypochlorite (HClO/ClO-) is a reactive oxygen species (ROS) produced by the enzyme myeloperoxidase (MPO), which catalyzes the reaction of hydrogen peroxide (H2O2) and chloride ion (Cl-), and plays an important role in the immune response to defend against invading pathogens. Excessive generation of hypochlorite could lead to oxidative stress, which causes inflammation and can lead to a range of diseases, including cardiovascular diseases, atherosclerosis, osteoarthritis, neuron degeneration and cancers.
BioTracker Rd1 Hypochlorite Live Cell Dye is a mitochondria-immobilized turn-on green fluorescent probe for the selective detection of hypochlorite (ClO-). The probe contains a benzoyl acetohydrazide linking site for ClO- recognition, a triphenylphosphonium group for mitochondria targeting, and a reactive methoxymaleimide subunit for mitochondrial fixation. The targeting and fixed properties of the probe enable it to accumulate in mitochondria and prevents leakage of the dye upon the decrease of mitochondrial membrane potential (MMP). This probe may be used to visualize exogenous and endogenous ClO- levels in living cells, tissues, and zebrafish.
Reference:
1. Li, M. Y., et. al. (2020). Mitochondria-Immobilized Fluorescent Probe for the Detection of Hypochlorite in Living Cells, Tissues, and Zebrafishes. Analytical chemistry, 92(4), 3262-3269.
Application
Purity ≥ 98% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.
Features and Benefits
Mitochondria-immobilized turn-on green fluorescent probe for the selective detection of hypochlorite.
Physical form
Lyophilized. Off-white solid.
Preparation Note
Store at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
Other Notes
Live cell fluorescent imaging
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| assay | ≥98% (HPLC) |
| form | lyophilized |
| mol wt | calculated mol wt 1080.94 |
| packaging | pkg of 1 mg |
| manufacturer/tradename | Millipore |
| storage condition | desiccated,protect from light |
| technique(s) | cell based assay: suitable |
| color | off-white |
| detection method | fluorometric |
| shipped in | ambient |
| storage temp. | −20°C |
