General description
Neuronal acetylcholine receptor subunit alpha-7 (UniProt: P36544; also known as nAChR7, Nicotinic acetylcholine receptor subunit alpha-7) is encoded by the CHRNA7 (also known as NACHRA7) gene (Gene ID: 1139) in human. CHRNA7 encodes the alpha-7 subunit of the neuronal nicotinic acetylcholine receptor (nAChR), which is part of a superfamily of ligand-gated ion channels. These receptors mediate fast synaptic signal transmission and play critical roles in neuronal communication, plasticity, and cognitive function. The alpha-7 subunit forms homopentameric receptors that are highly permeable to calcium ions and characterized by rapid desensitization. These receptors are activated by the neurotransmitter acetylcholine and other ligands, facilitating ion flux that influences neuronal excitability and signaling. CHRNA7 is ubiquitously expressed in both the central nervous system (CNS) and peripheral tissues. Within the CNS, these receptors are particularly abundant in regions involved in learning, memory, and sensory processing, such as the hippocampus and cortex. The alpha-7 nAChRs interact with various neurotransmitter systems and are integral to pathways such as Acetylcholine binding and downstream events, Regulating synaptic plasticity and excitatory neurotransmission. Mutations or deletions in CHRNA7 are linked to several neuropsychiatric and developmental disorders, including Chromosome 15q13.3 Deletion Syndrome which is associated with intellectual disability, epilepsy, and schizophrenia. Dysregulation of CHRNA7 signaling contributes to deficits in attention and memory. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Immunogen
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal extracellular domain of human CHRNA7.
Application
Quality Control Testing
Evaluated by Western Blotting in IMR-32 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHRNA7 in IMR-32 cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CHRNA7 in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound with CHRNA7 peptide with at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1K16 is a ZooMAb® rabbit recombinant monoclonal antibody that detects CHRNA7. It targets an epitope within 24 amino acids from the N-terminal extracellular domain.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1K16, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 56.45 kDa,observed mol wt ~57 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | monkey |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | N-terminal extracellular domain |
| Protein ID accession no. | NP_000737 |
| UniProt accession no. | P36544 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... CHRNA7(1139) |
