General description
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . In guinea pig IgG is subdivided into-IgG1 and IgG2. IgG2 can bind to Fc receptor of macrophages and monocytes. Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody can be used in immunohistology (diluted 1:50). Goat anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody reacts specifically with guinea pig IgG.
Immunogen
Purified guinea pig IgG.
Application
Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody can be used in direct ELISA (diluted 1:30,000) and dot blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western blot analysis of SF9 cells was performed using alkaline phosphatase conjugated goat anti-guinea pig IgGat 1:10000 as the secondary.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | goat |
| Quality Level | 200 |
| conjugate | alkaline phosphatase conjugate |
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| clone | polyclonal |
| form | buffered aqueous glycerol solution |
| technique(s) | direct ELISA: 1:30,000,immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50,western blot: 1:30,000 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
