General description
Lacks the mitochondrial targeting sequence but maintains the ability to neutralize pro-apoptotic Bcl-2 family members.
This recombinant Bcl-w lacks the mitochondrial targeting sequence but maintains the ability to neutralize pro-apoptotic Bcl-2 family members.
Biochem/physiol Actions
Anti-apoptotic member of the Bcl-2 family that prevents release of cytochrome c from the mitochondrial intermembrane space into the cytosol; promotes cell survival.
Like Bcl-2 and Bcl-x, the Bcl-w protein promotes cell survival, in contrast to other close homologues, Bax and Bak, which facilitate cell death. Bcl-w is an anti-apoptotic member of the Bcl-2 family that prevents release of cytochrome c from the mitochondrial intermembrane space into the cytosol. It is required for normal sperm maturation. Natural Bcl-w contains a C-terminal mitochondrial targeting sequence.
Physical form
Supplied as a 0.2 μm filtered solution in 25 mM HEPES, pH 7.5, 0.1 M KCl, and 10% glycerol.
Analysis Note
Measured by its ability to inhibit caspase-8-cleaved human BID-mediated release of cytochrome c from isolated mouse liver mitochondria using ELISA.
| recombinant | expressed in E. coli |
| Quality Segment | 200 |
| assay | >95% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | 18 kDa by SDS-PAGE |
| UniProt accession no. | Q92843 |
| shipped in | wet ice |
| storage temp. | −20°C |
| Gene Information | human ... BCL2L2(599) |
