产品介绍
Product Description
|
规格 |
|
| Bacterial or Yeast Strain: | BL21(DE3)pLysE, BL21(DE3) |
| Blue/White Screening: | No |
| Cloning Methylated DNA: | No |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Product Line: | One Shot™ |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | No |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^7 |
| Transformation Efficiency Level: | Subcloning Efficiency (10^6-10^7 cfu⁄µg) |
| Format: | One Shot |
| Improves Plasmid Quality: | No |
| Species: | E. coli |
|
描述 |
BL21(DE3)pLysE E. coli are ideal for use with T7 promoter-based expression vectors. Like BL21(DE3)pLysS E. coli, BL21(DE3)pLysE carry the lambda DE3 lysogen. In addition, BL21(DE3)pLysE cells contain the pLysE plasmid, which constitutively expresses T7 lysozyme. T7 lysozyme reduces the basal expression of target genes by inhibiting T7 RNA polymerase. The pLysE plasmid in BL21(DE3)pLysE expresses higher levels of T7 lysozyme than the pLysS plasmid in BL21(DE3)pLysS.
The BL21(DE3)pLysE strain provides tighter control of T7 RNA polymerase, which is necessary when the recombinant protein to be expressed is toxic.
