General description
Monocyte Chemotactic Protein-2 (MCP-2) belongs to a family of C-C chemotactic cytokines. MCP-1, -3, -4, and -5 are the other five monocyte chemo attractants identified. The MCP cytokines are involved in recruitment of leukocytes to the sites of inflammation, and activate NK cells and CD4+ and CD8+ T lymphocytes. Additionally, MCP cytokines can activate basophils to secrete histamines and also attract eosinophils. MCPs play an important role in pathological conditions such as systemic sclerosis, myocardia and cardiac ischemia. Anti-monocyte chemotactic protein 2 antibody specifically reacts with human MCP-2. The antibody shows <20% cross reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.
Immunogen
recombinant human MCP-2 expressed in E. coli.
Application
Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody may be used in ELISA at a concentration of 2.0 μg/ml. The antibody is suitable for neutralization reactions; ND50 is 1-4 μg/ml.
Biochem/physiol Actions
Neutralizes the biological activity of recombinant human MCP-2. Shows <20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose
Preparation Note
Purified using protein A.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 35509, monoclonal |
| form | lyophilized powder |
| species reactivity | human |
| technique(s) | indirect ELISA: suitable,neutralization: suitable |
| isotype | IgG1 |
| UniProt accession no. | P80075 |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... CCL8(6355) |
