General description
The epithelial cell adhesion molecule (EpCAM) is mapped to human chromosome 2p21 and has nine exons. EpCAM is a 40 kDa transmembrane glycoprotein present on cell surface. It has an intracellular domain called the EpICD, comprising of 26-amino acids. The extracellular domain (EpEX) has an epidermal growth factor (EGF) and a thyroglobulin repeat-like domain.
Immunogen
human breast carcinoma cell line MCF-7
Biochem/physiol Actions
The epithelial cell adhesion molecule (EpCAM) is highly expressed in carcinomas. It regulates cell signalling, proliferation, migration and adhesion. Proteolytic cleavage of EpICD domain is crucial for tumor progression. EpCAM is highly expressed in breast cancer and is considered as a potential target for gene therapy. Deletions in the 3′ region of the EpCAM gene is associated with colorectal cancer based lynch syndrome. Single nucleotide polymorphism in EpCAM gene is associated with infant diarrheal disorder, congenital tufting enteropathy(CTE).
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | Ber-EP4, monoclonal |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | ~1 mg/mL |
| technique(s) | immunofluorescence: 1-2 μg/mL using human breast adenocarcinoma MCF-7 cell line.,immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections.,immunoprecipitation (IP): suitable |
| isotype | IgG1 |
| UniProt accession no. | P16422 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... EPCAM(4072) |
