Application
Tumor Necrosis Factor Soluble Receptor I (TNF sRI) from mouse plays a pivotal role in the signaling of apoptosis and NF-κB activation because of its “death domain” present at the intracellular portion.
Biochem/physiol Actions
Tumor Necrosis Factor Soluble Receptor I (TNF sRI) from mouse belongs to TNF family of receptors and has a pivotal role in neutralizing the biological activities of both TNF-α and TNF-β. Soluble tumor necrosis factor receptor along with intrathecal interleukin-1 receptor antagonist shows an anti-allodynic action in rat L5 spinal nerve transection model of neuropathic pain.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 2.5 mg bovine serum albumin
Analysis Note
The biological activity is measured by its ability to inhibit the recombinant mouse TNF-α mediated cytotoxicity in mouse L929 cells, in the presence of the metabolic inhibitor actinomycin D.
| biological source | mouse |
| Quality Segment | 200 |
| recombinant | expressed in E. coli |
| assay | >97% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.5-1.5 μg/mL ED50 |
| mol wt | 21 kDa |
| packaging | pkg of 50 μg |
| storage condition | avoid repeated freeze/thaw cycles |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | P06804 |
| storage temp. | −20°C |
| Gene Information | mouse ... Tnfrsf1a(21937) |
