Anti-MAFF (C-Terminal) antibody produced in rabbit，200UL，M8194-200UL，Sigma

General description

The MAFF gene is located on the human chromosome at 22q13.1. The encoded protein is a small MAF protein and is expressed in the nucleus.
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) is a transcription factor which does not have a transactivation domain but possesses a leucine-zipper.

Immunogen

synthetic peptide corresponding to amino acids 147-163 of human MAFF (isoform a). The immunizing peptide does not cross-react with other members of the family.

Application

Anti-MAFF (c-terminal) antibody produced in rabbit has been used in:

• western blotting
• chromatin immunoprecipitation (chip)
• co-immunoprecipitation
• immunoprecipitation

Biochem/physiol Actions

Anti-MAFF (C-terminal) specifically recognizes MAFF.
The small MAF proteins are associated with many physiological processes including development, differentiation, hematopoiesis and stress response. Interestingly, these proteins regulate the stress response via different mechanisms. The MAFF gene is down-regulated in diabetes mellitus 1 patient. This gene is underexpressed in acute lymphoblastic lymphoma (ALL) patients.
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) has been shown to interact with other transcription factors and it associates with certain regulatory sequences in the gene encoding the oxytocin receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.