Application
TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line.
Biochem/physiol Actions
TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells.
Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
Analysis Note
Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D.
| biological source | human |
| Quality Segment | 200 |
| recombinant | expressed in NSO cells |
| description | N-terminal 6X histidine-tagged extracellular domain |
| assay | >97% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | 24 kDa by SDS-PAGE,predicted mol wt ~21 kDa |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | P50591 |
| storage temp. | −20°C |
| Gene Information | human ... TNFSF10(8743) |
