General description
A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.
Biochem/physiol Actions
Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Physical form
A 5 mM (1 mg in 550 µl) solution in DMSO.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
Legal Information
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
| Quality Segment | 100 |
| assay | ≥98% (HPLC) |
| form | solution |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,protect from light |
| color | pale yellow |
| fluorescence | λex ~495 nm,λem ~515 nm |
| storage temp. | −20°C |
| SMILES string | Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)O)Oc5c3ccc(c5)O |
| InChI | 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2 |
| InChI key | LTYUPYUWXRTNFQ-UHFFFAOYSA-N |
