General description
MTSEA biotin-XX is a membrane-impermeant crosslinker consisting of biotin-coupled 2-aminoethyl-methanethiosulfonate (MTSEA).
Application
MTSEA-Biotin-XX may be used to covalently label cysteine residues in proteins or thiol-containing molecules with a biotin tag for streptavidin-based detection, purification, or accessibility studies and, for biotinylation of 4TU‐labeled RNAs in direct RNA sequencing.
Biochem/physiol Actions
MTSEA-Biotin-XX selectively and efficiently labels cell surface thiols, including cysteine residues and specific RNA modifications (s4U and dm6A), forming disulfide bonds with enhanced reactivity. The extended -XX- linker facilitates optimal biotin-streptavidin interaction, while the overall structure allows for high-yield, rapid reactions. Biotin-XX-MTSEA enables imaging, purification, and sequencing applications through its ability to tag target molecules via methanethiosulfonate (MTS) reactions.
Disclaimer
Hygroscopic, Store with desiccant
| Quality Segment | 100 |
| assay | ≥98% (HPLC) |
| form | powder |
| storage condition | desiccated |
| color | white to beige |
| solubility | DMSO: 2 mg/mL, clear (warmed) |
| shipped in | wet ice |
| storage temp. | -10 to -25°C |
