General description
Succinate receptor 1 (UniProt: Q9BXA5; also known as G-protein coupled receptor 91, P2Y purinoceptor 1-like) is encoded by the SUCNR1 (also known as GPR91) gene (Gene ID: 56670) in human. Succinate receptor is a multi-pass membrane glycoprotein that belongs to the G-protein coupled receptor family with seven transmembrane domains. It serves as a receptor for succinate. SUCNR1 intracellular signaling is depended on the associated subunit type (G i, G s, and G q). Its expression is observed in kidney. Within the adaptive immune system, it has been detected in CD4+ and CD8+ T cells and in all subsets of B cells. T cells from patients with systemic lupus erythematosus (SLE) are reported to release large amounts of IL-10 and succinate following activation with oxidized mitochondrial DNA. Although its expression in the healthy CNS is reported to be low, its activation is considered as a key in the response of CNS cells to hypoxic-ischemic injury and inflammatory damage. In rodent retinal
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ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the third cytoplasmic domain within the C-terminal half of human Succinate receptor 1 (SUCNR1/GPR91).
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in Human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected SUCNR1/GRP91 in Human breast cancer tissue sections.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound SUCNR1/GPR91 peptide with a KD of 3.5 x 10^-8 in an affinity binding assay.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SUCNR1/GPR91 in human adipose tissue lysates.
Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected SUCNR1/GPR91 in SUCNR1 and GST Proteins.
Immunofluorescence Analysis: A 1:5,000 dilution from a representative lot detected SUCNR1/GPR91 in Human liver and porcine kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 1N21 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Succinate receptor 1 (SUCNR1/GPR91). It targets an epitope within 18 amino acids from the third cytoplasmic domain in the C-terminal half.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1N21, recombinant monoclonal |
| description | recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 38.7 kDa |
| purified by | using Protein A |
| species reactivity | porcine, human |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | ELISA: suitable,affinity binding assay: suitable,immunofluorescence: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_149039 |
| UniProt accession no. | Q9BXA5 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... SUCNR1(56670) |
