General description
HumanKine interleukin-23 (IL-23) is expressed as a heterodimeric 55 kDa cytokine composed of two glycosylated and disulfide linked subunits (p40 cystine linked to p19). Production in human HEK 293 cells offers authentic glycosylation. Cytokines produced in Sf9 or Sf21 cells have post-translational modifications, which are not human-like.
Interleukin-23 (IL-23) is a pro-inflammatory cytokine. It is mostly synthesized by activated dendritic cells and macrophages. It is a 20.7kDa protein with 189 amino acids. IL-23 is mostly expressed on activated/memory T cells and natural killer (NK) cells. This gene is located on human chromosome 12q13.3.
Biochem/physiol Actions
Interleukin-23 (IL-23) plays a major role in cutaneous immune-mediated inflammatory diseases like psoriasis, allergic contact dermatitis and atopic dermatitis. It is involved in cellular immunity, driving the expansion, stabilization and survival of Th17 cells.
Analysis Note
The specific activity was determined by the dose-dependent secretion of h-IFN-γ or IL-8 from human PBMC.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in HEK 293 cells |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| potency | ≤2 ng/mL EC50 |
| quality | endotoxin tested |
| mol wt | dimer 55 kDa (glycosylated) |
| packaging | pkg of 5X10 μg,pkg of 10 μg,pkg of 5 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | ≤1EU/mg |
| UniProt accession no. | Q9NPF7 |
| storage temp. | −20°C |
| Gene Information | human ... IL23A(51561) |
