General description
Interleukin-11 (IL-11) is synthesized by many mesenchymal origin cells like keratinocytes, synoviocytes, fibroblasts, bone marrow stromal cells, osteoblasts, and chondrocytes.
The gene IL-11 (interleukin 11) is mapped to human chromosome 19q13.4. It is a secreted protein.
Biochem/physiol Actions
Interleukin 11 (IL-11) is involved in promoting tissue fibrosis and regulates the function of chondrocytes, synoviocytes, and B cells. It is also involved in bone metabolism and tissue remodeling.
Interleukin-11, also called adipogenesis inhibitory factor (AGIF), is a pleotropic cytokine that acts on hematopoietic progenitor cells and stromal cells. Although recombinant human IL-11 has a predicted molecular mass of 19 kDa, it migrates as a 23 kDa band in SDS-PAGE. It is a functional homolog of IL-6, inducing the proliferation of certain plasmacytoma cell lines and acute phase protein secretion in the liver. IL-11 stimulates the production of erythrocytes, megakaryocytes, and T cell development of antibody producing B cells.
Physical form
Lyophilized from a 0.2 μm filtered solution of PBS and EDTA with BSA as a carrier protein.
Analysis Note
The biological activity is measured in a cell proliferation assay using T11, a subline of the IL-6-dependent murine plasmacytoma cell line T1165.85.2.1 that has been adapted to grow in IL-11.
| biological source | human |
| Quality Level | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| assay | >97% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.06-0.24 ng/mL ED50 |
| mol wt | 23 kDa |
| packaging | pkg of 5 μg |
| technique(s) | cell culture | mammalian: suitable |
| impurities | endotoxin, tested |
| UniProt accession no. | P20809 |
| storage temp. | −20°C |
| Gene Information | human ... IL11(3589) |
