General description
The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region.
Immunogen
peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in monkey, bovine and dog and differs by 2 amino acids in mouse and rat.
Application
Anti-ALIX antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.
Biochem/physiol Actions
ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X binds to endophilins, proteins that regulate membrane shape during endocytosis. Over-expression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.
PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 200 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | buffered aqueous solution |
| mol wt | antigen ~95 kDa |
| species reactivity | mouse, human |
| concentration | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of human Jurkat cells.,western blot: 2.5-5 μg/mL using whole extracts of mouse A20 cells. |
| UniProt accession no. | Q8WUM4 |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PDCD6IP(10015) mouse ... Pdcd6ip(18571) |
