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| Description: | DyLight 680 Antibody Labeling Microscale Kit |
| Excitation/Emission: | 692⁄712 |
| Labeling Scale: | 100 µg |
| Labeling Target: | Antibodies (General), Proteins (General) |
| Product Line: | DyLight™ |
| Label or Dye: | DyLight™ 680 |
| Quantity: | 5 rxn kit |
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Sufficient For: Labeling and purifying 5 x 100 µg (1 mg/mL) of IgG or other proteins • DyLight Fluor NHS Ester, 5 x 15 µg vials • Borate Buffer (0.67M), 1 mL • Purification Resin, 5 mL • Microcentrifuge Spin Columns, 5 columns • Microcentrifuge Collection Tubes, 10 tubes Store the DyLight NHS Ester at -20°C. Store all other kit components at 4°C. |
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描述 |
The Thermo Scientific DyLight 680 Microscale Antibody Labeling Kit contains an NHS ester-activated derivative of high-performance DyLight 680 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 µg of IgG—the amine-reactive DyLight 680 NHS-ester in convenient single-use vials, as well as purification resin and spin columns for the preparation of ready-to-use conjugate..
DyLight 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5.5™ dye and Alexa Fluor™ 680, and is ideal for multi-color applications.The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 680 Amine-Reactive Dye is also available as a stand-alone reagent.
Features of DyLight 680 NHS-Ester:
• High performance—DyLight 680 fluoresces brighter than Alexa Fluor 680 and Cy5.5 dye
• Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
• Convenient kit sizes—standard and microscale sizes are offered to match your experimental needs
• Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling
Applications:
• Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
• Target protein labeling for in vitro and in vivo fluorescent detection strategies
DyLight 680 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.
Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 680 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.
Related Products
DyLight™ 680 NHS-Ester
DyLight™ 680 Antibody Labeling Kit
