General description
Tyrosine-protein kinase JAK3 (UniProt: P52333; also known as EC:2.7.10.2, Janus kinase 3, JAK-3, Leukocyte janus kinase, L-JAK) is encoded by the JAK3 gene (Gene ID: 3718) in human. JAK/STAT signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia. JAK3 is a non-receptor tyrosine kinase that mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. It also mediates the secretion of IL-2, IL-4, and IL-9 that have been implicated in allergic inflammation. In the cytoplasm, it plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma, which is a component of the IL-2 receptor and other interleukin receptors. Following ligand binding to cell surface receptors, it phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STAT proteins. Subsequently, it phosphorylates STAT proteins following their recruitment to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. JAK3 possesses two phosphotransferase domains (aa 521-781 and 822-1111) and the second domain contains the catalytic region. JAK3 undergoes tyrosine phosphorylation on residues 980 and 981 in response to IL-2 and IL-4 and is dephosphorylated by protein tyrosine phosphatase non-receptor type 2 (PTPN2) that negatively regulates cytokine-mediated signaling. In humans, mutations in JAK3 gene have been associated with severe combined immunodeficiency disease. Three isoforms of JAK3 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Remenyi, J., et al. (2021). Sci. Rep. 11; Article 10093; Saharinen, P., and Olli Silvennoinen, O. (2002). J. Biol. Chem. 277(49); 47954-47963; Johnston, JA., et al. (1994). Nature. 370(6485); 151-153).
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Immunogen
KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphotyrosine 980/981 from human JAK3.
Application
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected JAK3 in HepG2 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-JAK3 (Tyr 980/981) in HepG2 cells.
Affinity Binding Assay:: A representative lot of this antibody bound phospho-Jak3 (Tyr980/981) peptide with a KD of 9.2 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 1 µg from a representative lot detected phospho-JAK3 (Tyr980/981) in one million NK-92 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone 2D9 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects JAK3 phosphorylated on tyrosine 980/981.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Preparation Note
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 200 |
| recombinant | expressed in HEK 293 cells |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| description | clone 2D9 ,recombinant, expressed in HEK 293 cells |
| product line | ZooMAb® learn more |
| form | lyophilized |
| mol wt | calculated mol wt 125.1 kDa,observed mol wt ~110 kDa |
| purified by | using Protein A |
| species reactivity | human |
| species reactivity (predicted by homology) | canine, monkey, porcine |
| packaging | antibody small pack of 25 |
| greener alternative product characteristics | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation |
| sustainability | Greener Alternative Product |
| technique(s) | affinity binding assay: suitable,flow cytometry: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| epitope sequence | C-terminal half |
| Protein ID accession no. | NP_000206 |
| UniProt accession no. | P52333 |
| greener alternative category | Aligned |
| shipped in | ambient |
| storage temp. | 2-8°C |
| Gene Information | human ... JAK3(3718) |
