规格
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Assay:
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Phosphatase Assay |
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Excitation/Emission:
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358⁄452 |
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Product Line:
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EnzChek®, RediPlate™ |
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Shipping Condition:
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Room Temperature |
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Substrate:
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DiFMUP |
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Substrate Properties:
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Chemical Substrate |
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Substrate Type:
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Phosphatase Substrate |
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Target Enzyme:
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Serine⁄Threonine Phosphatases |
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Technique:
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Fluorescence Intensity |
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Detection Method:
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Fluorescent |
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For Use With (Equipment):
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Microplate Reader |
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Label or Dye:
|
DiFMU |
储存
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Store in freezer (-5 to -30°C) and protect from light. |
The RediPlate™ 96 EnzChek® Serine/Threonine Phosphatase Assay Kit provides a fast, simple and direct fluorescence-based assay for detecting serine/threonine phosphatases and their corresponding modulators and inhibitors. Unlike other microplate assays, this kit provides the necessary reagents predispensed into a 96-well microplate. Simply reconstitute the fluorogenic substrate in the assay wells with buffer, add the desired sample to the wells, incubate and then quantitate the fluorescence in any standard fluorescence-based microplate reader. Inhibitors are included in each assay well to ensure that the assay is selective for Ser/Thr PPases- other phosphatases, including tyrosine phosphatase do not significantly react with the substrate. Additional advantages of this assay include compatibility with non-ionic detergents and insensitivity to free phosphate.
