规格
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Delivery Type:
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Transfection |
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Key Functions:
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Reporter Assays |
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Product Line:
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Pierce™ |
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Promoter:
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TK |
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Reporter Gene:
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Gaussia Dura Luciferase |
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Selection Agent (Eukaryotic):
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Puromycin |
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Cloning Method:
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Restriction Enzyme ⁄ MCS |
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Description:
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pTK-Gaussia-Dura Luc Vector |
储存
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Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C. |
The Thermo Scientific pTK-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the weak Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Gaussia-Dura Luc vector:
• Weak (TK) constitutive promoter for co-transfection and normalization
• Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
• Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication origin
Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
These vectors are subject to a limited use label license.
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pMCS-Gaussia-Dura Luc Vector for Luciferase Assays
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