产品介绍
Product Description
|
规格 |
|
| Concentration Ratio: | 1 :1 |
| Form: | Liquid |
| Glutamine: | No Glutamine |
| HEPES Buffer: | No HEPES |
| Phenol Red Indicator: | No Phenol Red |
| Shipping Condition: | Room Temperature |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Concentration: | 1 X |
| Sterility: | Sterile |
|
储存 |
|
| Store in refrigerator (2–8°C) and protect from light. |
|
描述 |
William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco® William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.
This William's E Medium is modified as follows:
| Without |
| • Phenol Red |
| • L-glutamine |
| • HEPES |
The complete formulation is available.
Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate
Dual-Site cGMP Manufacturing
For supply chain continuity, we manufacture Gibco® William's E Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
