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规格 |
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| Compatible Cells: | Mammalian Cells, Eukaryotic Cells |
| Detection Method: | Fluorescent |
| For Use With (Equipment): | Fluorescence Microscope |
| Format: | Dropper Bottle(s) |
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储存 |
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3 x 2.5 mL NucBlue® Live 3 x 2.5 mL NucGreen® Dead Store at 2–8°C, or room temperature. |
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描述 |
ReadyProbes® Cell Viability Imaging Kit (Blue/Green) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of room temperature, stable NucBlue® Live reagent (Hoechst 33342) and NucGreen® Dead reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue® Live reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. NucGreen® Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC/GFP (green) filter set. This kit is appropriate for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.
NucBlue® Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460 nm)
NucGreen® Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC/GFP (green) filter set (excitation/emission maxima: 504/523 nm)
See other ReadyProbes® reagents for cell staining
Learn more about other assays for cell viability
Suggestions for use
• NucBlue® Live and NucGreen® Dead reagents may be added directly to cells in full growth media or a compatible buffer solution.
• In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
