规格
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Final Product Type:
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Total RNA, Transcriptome RNA, micro RNA |
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For Use With (Application):
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Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting, Cloning |
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Isolation Technology:
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Organic Extraction |
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No. of Reactions:
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200 Preps |
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Quantity:
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1 |
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Sample Type:
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Bacteria (Gram-Negative), Bacteria (Gram-Positive) |
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Yield:
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70 µg |
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High-throughput Compatibility:
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Not High-throughput Compatible (Manual) |
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Purification Target:
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Total RNA, Transcriptome RNA, miRNA |
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Purification Time:
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1 hr |
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Scale:
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Scaleable |
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Starting Material Amount:
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Up to 1 x 10^8 cells |
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System Type:
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TRIzol |
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Product Line:
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TRIzol® |
储存
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Includes 200mL TRIzol® Reagent and 2 x 20 mL Max Bacterial Enhancement Reagent.
Store TRIzol® Reagent at 2°C to 25°C. Max Bacterial Enhancement Reagent may be stored at room temperature. |
The TRIzol® Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol® Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.
Key Features of the TRIzol® Max™ Bacterial RNA Isolation Kit:
• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps
Two Products in One Kit
The TRIzol® Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol® Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol® Reagent generates a high-quality, minimally degraded RNA product.
Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol® Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
